含非洲马瘟病毒部分核酸序列的病毒样颗粒的研制

doi:10.11843/j.issn.0366-6964.2015.04.018

Abstract

Abstract:

This experiment was conducted to prepare the virus-like particles containing the partial gene fragment of African horse sickness virus (AHSV).Genome segment 7 (S7) encoding protein VP7，the group-specific protein of AHSV，is a primary target for reverse transcription-PCR and real time reverse transcription-PCR detection of AHSV.The conservative sequence of S7 gene was synthesized and cloned into plasmid pNH-MS2his，which contained the coat protein gene of E.coli bacteriophage MS2 and His tag，to construct the prokaryotic express vector pNH-MS2his-VP7.The recombinant plasmid pNH-MS2his-VP7 was transformed into BL21 (DE3).Expression of the target protein was induced by IPTG for 5 h.The virus-like particles containing the S7 RNA sequence of AHSV were simply obtained by induction and sequent Ni+ chromatography purification.The virus-like particles were confirmed have good uniformity and stability.They have great prospect as the standard and quality control in the detection of AHSV.

CLC Number:

S852.659.4

SUN Min，LIANG Cheng-zhu，WANG Qun，ZHANG Xiao-wen，XIAO Xi-zhi，GENG Juan，CHEN Ji-xiang. Preparation of the Virus-like Particles Containing the Partial Gene Fragment of AHSV[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 637-643.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.04.018

https://www.xmsyxb.com/EN/Y2015/V46/I4/637

References

［1］世界动物卫生组织.陆生动物诊断试验和疫苗手册［M］.2007.
World Organisation for Animal Health.Manual of diagnostic tests and vaccines for terrestrial animals［M］.2007.(in Chinese)
［2］赵文华，杨仕标，王金萍，等.非洲马瘟病毒群特异性RT-PCR检测方法的研究［J］.生物技术通报，2011（4）：204-207.
ZHAO W H，YANG S B，WANG J P，et al.Development of RT-PCR method for detection of AHSV［J］.Biotechnology Bulletin，2011（4）：204-207.(in Chinese)
［3］曾昭文，花群义，段纲.非洲马瘟病毒分子生物学研究进展［J］.动物医学进展，2006，27（10）：1-6.
ZENG Z W，HUA Q Y，DUAN G.Advance in molecular biology of African Horse Sickness Virus［J］.Progress in Veterinary Medicine，2006，27（10）：1-6.(in Chinese)
［4］高志强，张鹤晓，赖平安，等.非洲马瘟病毒VP7基因拼接、表达及重组ELISA方法的建立与初步应用［J］.畜牧兽医学报，2008，39（11）：1548-1553.
GAO Z Q，ZHANG H X，LAI P A，et al.Development and application of the recombinant ELISA based on the assembling and expression of VP7 gene of African horse sickness virus［J］.Acta Veterinaria et Zootechnica Sinica，2008，39（11）：1548-1553.(in Chinese)
［5］张海明，段晓冬，相文华，等.非洲马瘟诊断技术研究概况［J］.动物医学进展，2013，34（6）：175-178.
ZHANG H M，DUAN X D，XIANG W H，et al.Progress on diagnostic techniques for African Horse Sickness［J］. Progress in Veterinary Medicine，2013，34（6）：175-178.(in Chinese)
［6］中国计量科学研究院.JJF 1343—2012标准物质定值的通用原则及统计学原理［S］.北京：中国标准出版社，2012.
National Institute of Metrology.JJF 1343-2012 general and statistical principles for characterization of reference materials［S］.Beijing：Standards Press of China，2012.(in Chinese)
［7］ZHAN S，LI J M，XU R H，et al.Armored long RNA controls or standards for branched DNA assay for detection of human immunodeficiency virus type 1［J］.J Clin Microbiol，2009，47(8)：2571-2576.
［8］STEVENSON J，HYMAS W，HILLYARD D.The use of armored RNA as a multi-purpose internal control for RT-PCR ［J］. J Virol Methods，2008，150（1-2）：73-76.
［9］CHENG Y J，NIU J J，ZHANG Y Y，et al.Preparation of His-tagged armored RNA phage particles as a control for real-time reverse transcription-PCR detection of severe acute respiratory syndrome coronavirus ［J］. J Clin Microbiol，2006，44(10)：3557-3561.
［10］WEI Y X，YANG C M，WEI B J，et al.RNase-resistant virus-like particles containing long chimeric RNA sequences produced by two-plasmid coexpression system ［J］.J Clin Microbiol，2008，46(5)：1734-1740.
［11］窦敏，张国广，于广福，等.含口蹄疫病毒IRES RNA病毒样颗粒表达载体的构建［J］.中国生物工程杂志，2007，27（9）：31-35.
DOU M，ZHANG G G，YU G F，et al.Construction of the expression vector of virus-like particles containing FMDV IRES RNA［J］.China Biotechnology，2007，27（9）：31-35.(in Chinese)
［12］彭建明，李金明，徐克前，等.含人甲胎蛋白mRNA部分序列的耐核糖核酸酶病毒样颗粒的构建和表达［J］.中华肝脏病杂志，2005，13(4)：304-306.
PENG J M，LI J M，XU K Q，et al.Construction and expression of RNase-resisting virus-like particles containing partial sequence of alpha-fetoprotein messenger RNA［J］.Chinese Journal of Hepatology，2005，13(4)：304-306.(in Chinese)

[1]	CHEN Shuyu, ZHU Xuejiao, ZHOU Jinzhu, TAO Ran, ZHANG Xuehan, Suolangsizhu, Gongga, LI Bin. Inhibition of Porcine Rotavirus in vitro Replication by Guanylate-binding Proteins GBP1 and GBP2 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 245-257.
[2]	LI Huihui, DONG Keer, LIU Xinbo, ZHANG Chunxiao, MA Chao, CHEN Liping, ZHONG Qi, YAO Gang, MA Xuelian. Establishment and Application of Rapid Detection Method for Bovine Norovirus and Bovine Rotavirus Dual RAA-LFD [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 406-412.
[3]	LI Xiaohan, YANG Fuchun, LIU Rui, GAO Li, CUI Hongyu, ZHANG Yanping, LIU Changjun, QI Xiaole, WANG Xiaomei, GAO Yulong, LI Kai. Construction of Recombinant Herpesvirus of Turkey Expressing Infectious Bursal Disease Virus VP2 Gene and Analysis of Its Biological Characteristics [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 656-662.
[4]	LI Zhanhong, ZHU Pei, SONG Ziang, LI Zhuoran, YANG Zhenxing, LI Huachun, YANG Heng, LIAO Defang. Establishment and Application of a Group Specific Reverse Transcriptase Loop-mediated Isothermal Amplification Method of Bluetongue Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2244-2253.
[5]	LÜ Ting, CHEN Hongyin, TANG Cheng, YUE Hua. Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2361-2368.
[6]	LI Zhanhong, SONG Ziang, LIAO Defang, YANG Zhenxing, XIE Jiarui, GAO Xiang, HU Zhongyan, LI Zhuoran, LI Huachun, YANG Heng. Whole Genome Sequence Analyses of Bluetongue Virus Serotype 7 Strain Isolated from Yunnan Province [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1337-1348.
[7]	LI Maolin, ZHANG Qijin, XU Qingyuan. Global Distribution of Bluetongue [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 881-890.
[8]	YI Huashan, ZHAO Yao, MA Xianping, LI Huan, XIA Yu, ZHU Wenqing, LIU Qianru, CHEN Siqian, CAO Huizhen, YAO Ting, GAO Lixu, ZHANG Jinyang, YANG Fahui, WEI Xiaorong, LI Qianyong, XIE Yuanbing. Establishment and Preliminary Application of Indirect ELISA Based on Recombinant NS4 Protein for Detection of Bluetongue Virus Antibodies [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3133-3140.
[9]	YU Kexiang, LIU Cunxia, GONG Xiao, LU Xiao, HU Feng, GUO Xiaozhen, MA Xiuli, LI Yufeng, HUANG Bing, SONG Minxun. Isolation and Identification of a Variant Strain of Reovirus from Broilers [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1039-1047.
[10]	LI Ran, TANG Cheng, LUO Xue, YUE Hua. Isolation and Gene Sequence Analysis of G6P[11] Bovine Rotavirus HY-1 Strain from Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 592-601.
[11]	YANG Zhenxing, MENG Jinxin, XIAO Lei, ZHU Jianbo, LIAO Defang, GAO Lin, LI Zhanhong, YANG Heng, LI Huachun. Isolation of Epizootic Haemorrhagic Disease Virus Serotype 7 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 602-610.
[12]	LI Zhanhong, WANG Jinping, YANG Heng, LIAO Defang, SONG Jianling, GAO Lin, HE Yuwen, LI Huachun. Isolation of Bluetongue Virus Serotype 9 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 354-363.
[13]	YANG Heng, XIAO Lei, LI Zhan-hong, MENG Jin-xin, YANG Zhen-xing, Lü Min-na, LIN Xu-hui, LIAO De-fang, NIU Bao-sheng, LI Hua-chun. Sequence Analysis of Palyam Serogroup Virus Isolated in South China from 2012 to 2016 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 761-770.
[14]	CHI Xiao-juan, SHAO Wen-han, CHEN Ji-long, CHEN Yue-xiang, WANG Song. The Research Advance of Host Immune Response Induced by Avian-origin Reovirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2249-2257.
[15]	HUANG Li, XIE Zhi-xun, XIE Li-ji, HUANG Jiao-ling, XIE Zhi-qin, FAN Qing, DENG Xian-wen, LUO Si-si, ZHANG Yan-fang, ZENG Ting-ting, WANG Sheng, ZHANG Min-xiu. Transcriptomic Expression Profiles of LMH Cells Infected with Avian Reovirus Virulent Strain and Attenuated Vaccine Strain [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2135-2147.

Preparation of the Virus-like Particles Containing the Partial Gene Fragment of AHSV

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments